BIOLOGICAL STUDIES ON DIPHTHERIA TOXIN II. SKIN REACTION-INHIBITORY FACTOR
نویسندگان
چکیده
منابع مشابه
Studies on the Molecular Weight of Diphtheria Toxin, Antitoxin, and Their Reaction Products
Purified diphtheria antitoxic horse pseudoglobulin has been prepared which is homogeneous by sedimentation, diffusion, and electrophoresis. Immunologically, however, the preparation contains only 43.5 per cent antitoxin specifically precipitable by toxin. The inactive pseudoglobulin remaining after specific precipitation was found to have the same physical and chemical properties as the origina...
متن کاملStudies of the Activity of Diphtheria Toxin
It has been demonstrated that a saturating dose of diphtheria toxin produced a 90% inhibition of polio-virus replication in HeLa cells. This inhibition was reflected in infectious viral RNA synthesis and in mature virus production. Toxin had no direct effect on virus particles or I-RNA, and poliovirus adsorption and eclipse appeared to be carried out normally in intoxicated cells. When toxin wa...
متن کاملStudies on the Mode of Action of Diphtheria Toxin
Extracts from HeLa cells treated with excess diphtheria toxin for several hours, until all protein synthesis has been arrested, are still able to stimulate the poly U-directed incorporation of phenylalanine into polypeptides at a moderate rate. Activity may be restored to normal levels or above by addition of a soluble enzyme fraction containing transferase II. Our results are in agreement with...
متن کاملStudies on Peroxidative Detoxification of Purified Diphtheria Toxin
The mechanism of the peroxidative oxidation of diphtheria toxin has been investigated. It has been found that this reaction requires the presence of an oxidizable, dialyzable cofactor in addition to hydrogen peroxide and peroxidase. Cofactors are present in a variety of materials and have been partially purified from urine. The flocculation titer of toxin modified by peroxidation remains unchan...
متن کاملStudies on the Mode of Action of Diphtheria Toxin
The effect of crystalline diphtheria toxin on protein synthesis in vivo was evaluated in guinea pigs and mice. By two independent methods of analysis (microdensitometry of tissue radioautograms and radioactivity of tissue proteins), it was established that inhibition of protein synthesis was not a widespread metabolic effect of diphtheria toxin. In the sensitive guinea pig, only the heart and t...
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ژورنال
عنوان ژورنال: Japanese Journal of Medical Science and Biology
سال: 1962
ISSN: 0021-5112,1884-2828
DOI: 10.7883/yoken1952.15.97